Auxotrophic Mutant Strains of Rhizobium etli Reveal New Nodule Development Phenotypes
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چکیده
منابع مشابه
Ectopic Expression of the Rhizobium etli amtB Gene Affects the Symbiosome Differentiation Process and Nodule Development
Under conditions of nitrogen limitation, soil bacteria of the genus Rhizobium are able to induce the development of symbiotic nodules on the roots of leguminous plants. During nodule organogenesis, bacteria are released endocytotically inside the invaded plant cells where they differentiate into their endosymbiotic form called bacteroids. Bacteroids surrounded by a plant-derived peribacteroid m...
متن کاملCharacterization of a Rhizobium etli chromosomal gene required for nodule development on Phaseolus vulgaris L.
A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chromosomal fragment. The 3.6-kb fragment was subcloned from the cosmid pUHR80 whi...
متن کاملRhizobium etli asparaginase II
Bacterial L-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (AnsA) from Rhizobium etli and described an increasing type of AnsA family members...
متن کاملMultiresistance genes of Rhizobium etli CFN42.
Multidrug efflux pumps of bacteria are involved in the resistance to various antibiotics and toxic compounds. In Rhizobium etli, a mutualistic symbiont of Phaseolus vulgaris (bean), genes resembling multidrug efflux pump genes were identified and designated rmrA and rmrB. rmrA was obtained after the screening of transposon-generated fusions that are inducible by bean-root released flavonoids. T...
متن کاملA cysG mutant strain of Rhizobium etli pleiotropically defective in sulfate and nitrate assimilation.
By its inability to grow on sulfate as the sole sulfur source, a mutant strain (CTNUX8) of Rhizobium etli carrying Tn5 was isolated and characterized. Sequence analysis showed that Tn5 is inserted into a cysG (siroheme synthetase)-homologous gene. By RNase protection assays, it was established that the cysG-like gene had a basal level of expression in thiosulfate- or cysteine-grown cells, which...
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ژورنال
عنوان ژورنال: Molecular Plant-Microbe Interactions®
سال: 2002
ISSN: 0894-0282,1943-7706
DOI: 10.1094/mpmi.2002.15.5.501